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APPARATUS AND REAGENTS

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Reagents

0.018 N Sulphuric acid - dissolve 1.0ml concentrated sulphuric acid in 2.0 litres distilled water. Potassium dichromate solutions - weight accurately approximately 80 mg of potassium dichromate and dissolve it in 1.0 litre 0.018 N sulphuric acid.

Calculate its molarity as follows:

It should give a solution of approximately 0.25 mM. make two accurate, successive half dilutions of this solution with 0.018 N sulphuric acid to give solutions of approximately 0.125 mM and 0.0625 mM.

Acetic acid A.R.
Acetonitrile A.R.
Benzene A.R.
Absolute ethanol A.R.
Methanol A.R.
Chloroform A.R.
Ethanol A.R.

Apparatus

Spectrophotometer - capable of measurements from 200 - 400 nm.
Quartz - Faced cells - 1 cm.
Lens tissue.
Disposable pipettes and rubber teats.
Acetone wash bottle.
Beaker - 25 Oml, for waste solvent.
Volumetric flasks - 100, 250ml.

 

MYCOTOXIN STANDARDS

(a) Suppliers(a)

  1. Aldrich Chemical Co Ltd
    The Old Brickyard
    New Road
    Gillingham
    Dorset SP 8 4JL
    UK
  2. Sigma Chemical Co Ltd
    Fancy Road
    Poole
    Dorset BH17 7NH
    UK
  3. Phase Separations Ltd
    Deeside Industrial Estate
    Queensferry
    Clwyd CH5 2LR
    UK
  4. Uniscience Ltd
    Uniscience House
    8 Jesus Lane
    Cambridge CB5 8BA
    UK
  5. Makor Chemicals Ltd
    P.O.B. 6570
    Jerusalem 91060
    Israel
  6. Pierce and Warriner (UK) Ltd
    44 Upper Northgate Street
    Chester
    Cheshire CH1 4EF
    UK
  7. C.P. Laboratories Ltd
    (Calbiochem)
    PO Box 22
    Bishops Stortford
    Herts CM22 7RQ
    UK
  8. Pfaltz and Bauer, Inc.,
    375 Fairfield Avenue
    Stamford
    Connecticut 06902
    USA
  9. Sigma Chemical Co
    PO Box 14508
    St Louis
    Missouri 63178
    USA

 

Calbiochem Agencies in Middle East and Africa

Nigeria
Nigerian Hoechst Ltd
PO Box 261
Ikeja
Lagos

Lebanon
Hosta Pharma
B.P. 5767
R L Beyrouth
Liban

Kuwait
Bader Sultan and Bros Co
PO Box Safat 867
KWT - Kuwait it

If no agency is listed for a country order from C.P. Laboratories Ltd. UK.

 

Calbiochem Agencies in South East Asia and the Far East

Australia
Calbiochem - Behring
Australia Pty Ltd
PO Box 37
Carlingford
NSW 2118

Philippines
Hoechst Far East Marketing corp
Hoechst House
Salcedo Street
Legaspi Village
Makati, Metro Manila
PO Box 433
Commercial Centre 3117

Malaysia
Hoechst Malaysia Sdn Bhd
468-6C Jalan Ipoh
PO Box 540
Kuala Lumpur

Singapore
Hoechst Singapore Pte Ltd
Circuit Road
PO Box 89
Singapore 9137

Republic of Korea
Han-Dok Remedia Ind. co Ltd
344 Sangbong-dong
Dongdaemun - ku
C.P.O. Box 30
Seoul

Japan
Hoechst Japan Ltd
C.P.O. Box 1256
Tokyo

New Zealand
Hoechst New Zealand Ltd
PO Box 67
Auckland

 

Calbiochem Agencies in Central and South America

Brazil
Casa Fachada Ltda
Setor Diagnosticos
Rua Julio Verne 55
04725 Santo Amaro
Sao Paulo

Chile
Victor Manuel Grez O
Ahumada 312
Officina 721
Casilla 14656
Santiago

Colombia
Hoechst Colombiana S.A.
Apartado Aero 80188
Bogota

Costa Rica
Productos Hoechst Ltda
Apartado 10158
San Jose

Dominican Republic
Hoechst Dominicana S.A.
Apartado Postal 830
Santo Domingo

Ecuador
Hoechst - Eteco S.A.
Casilla 1408
Quito

Guatemala
Quimica Hoechst de Guatemala S.A.
Km. 15.5 Caratera Roosevelt
apartado 155
Guatemala, GA.

Venezuela
Hoechst Remedia S.A.
Apartado 80222
Caracas 108

 

(b) Source and approximate prices

1. Allatoxin B1(d) Amount(b) (mg) Approximate Cost(c) (£)
Available from all suppliers 5 10
10 15
50 70
250 320
NB It may be possible to obtain, free of charge, a solution in chloroform containing approximately 10 ug/ml aflatoxin B1 from: -
Dr P L Schuller    
Rijks Institut voor de Volksgesondheid    
Postbus 1, Bilthoven    
The Netherlands    
2. Aflaloxin B2(d)    
Available from all suppliers 5 18
  10 30
  50 150
3. Allatoxin G1(d)    
Available from all suppliers 5 17
  10 30
  50 125
4. Atlatoxin G2(d)    
Available from all supliers 5 20
  10 30
  50 150
5. Aflaloxin M1(d)    
Available from suppliers 0.01 20
(2) (4) (5) (6) (7) (9) 0.05 57
vacuum dried or as a 10 ug/ml 0.25 200
solution in chloroform    
6. Allatoxin M2(d)    
Available from suppliers 0.01 20
(2) (4) (5) (6) (9) vacuum dried or as a 10 ug/ml solution in chloroform 0.05 65
7. Aflatoxin Qualitative Standard Kit    
(Contains 10 mg each of B1, B2, G1, G2)   90-140
Available from suppliers    
(1) (2) (4) (5) (6) (9)    
8. Aflatoxin Quantitative Standard Kit    
(Contains Img each of B1, B2, G1, G2)   30
Available from suppliers    
(1) (2) (4) (5) (6) (9)    
9. Citrinin    
Available from suppliers 5 20
(1) (2) (4) (5) (7) (9) ) (9) 10 30
  25 40
  50 107
10. Cytochalasin A    
Available from suppliers 1 12
(1) (2) (4) (5) (7) (9) 5 30
  10 47
11. Cytochalasin B    
Available from suppliers 1 6
(1) (2) (4) (5) (7) (9) 5 20
  10 32
  50 135
12. Cytochalasin C    
Available from suppliers 1 14
(1) (2) (4) (5) (7) (9) 5 40
  10 65
13. Cytochalasin D    
Available from suppliers 1 13
(1) (2) (4) (5) (7) (9) 5 35
  10 65
14. Cytochalasin E    
Available from suppliers 1 10
(1) (2) (4) (5) (9) 5 30
  10 50
15. Diacetoxyscirpenol    
Available from suppliers 5 13
(2) (4) (5) (6) (7) (9) 10 25
  25 50
  50 95
16. Luleoskyrin    
Available from suppliers 5 25
(2) (4) (5) (6) (7) (9) 25 80
  100 280
17. Ochraloxin A    
Available from suppliers 5 30
(1) (2) (4) (5) (6) (7) (9) 25 120
18. Patulin    
Available from suppliers 10 26
(1) (2) (4) (5) (6) (7) (9) 25 35
  50 100
19. Penicillic Acid    
Available from suppliers 5 8
(1) (2) (4) (5) (6) (9) 10 12
  25 23
  100 75
20. Roridin A    
Available from suppliers 5 25
(2) (4) (5) (6) (9) 25 80
21. Rubratoxin B    
Available from suppliers 5 20
(1) (2) (4) (5) (6) (7) (9) 10 35
  25 64
22. Sterigmatocystin    
Available from suppliers 5 20
(1) (2) (4) (5) (6) (7) (9) 10 36
  25 100
  50 150
23. T-2 Toxin'    
Available from suppliers 5 24
(2) (4) (5) (6) (7) (9) 25 56-100
24. Verrucarin A    
Available from suppliers 1 7
(2) (4) (5) (6) (9) 5 25
  25 85
25. Verrucarol    
Available from suppliers 1 12
(2) (4) (5) (9) 5 50
26. Zearalenone    
Available from suppliers 10 5
(2) (4) (5) (9) 25 12
27. Mycoloxin Kits(f)    
Available from suppliers    
(2) (4) (5) (9)    
1. Ochratoxin 5mg, Patulin 10mg, Penicillic acid 10-25 mg, Rubratoxin 5-10mg.   86
2. Citrinin 10mg, Luteoskyrin 5mg, Sterigmatocystin 5mg, Zearalenone 10-25mg.   68
  1. The names and addresses of suppliers are given for convenience and it is not intended to imply that these are the only suppliers of mycotoxin standards.
  2. Not all suppliers can supply the amounts listed.
  3. An approximate cost is quoted for information. However, this can vary greatly between suppliers.
  4. Other aflatoxin derivatives are available from suppliers (2) (4) (5) (6) (9)
  5. Other T-2 derivatives are available from sup. pliers (2) (4) (5) (9).
  6. The actual amounts of each toxin obtained with the kit depends on the supplier.

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